10^th and 11^th October 2019 – Cryo-Electron Microscopy in structural biology: paving the way towards precision biomedicine and biotechnology. A conference in memory of Emilia Chiancone.

Rome, Italy
10^thOctober 2019 · Aula Odeion Sapienza
11^th October 2019 · Sala Convegni CNR

SESSIONS

Cryo-electron microscopy and biomedicine.
Cryo-electron microscopy sheds light on dynamical processes.
The methodology and its developments.
Round table on Cryo-electron microscopy: development of networks and infrastructures.

The Conference is intended to increase the awareness of the Sapienza, CNR and Italian scientific community of the most recent development in the determination of macromolecular and supramolecular complexes in precision medicine and rational drug design.

We wish to honor the memory of Prof. Emilia Chiancone, who championed an integrative approach to biomedicine through excellent biochemistry, integrative structural biology in the context of the synergy between Academia and CNR with a transnational and interdisciplinary vision.

The methodology of single particle cryo-electron microscopy is revolutionizing the understanding of physiology and pathology: the determination of the structure of the insulin-insulin receptor complex, of the telomere and of the ryanodine receptor, just to name a few, will allow us to design therapies with an unprecedented insight of their biological targets.

It is of crucial importance that the Italian larger public and scientific community achieve awareness of this scientific revolution, awarded the Nobel Prize in 2017.

Scientific Committee:
A. Boffi, B. Botta, L. Federici, A. Ilari, P. Lavia, F. Mancia, A.E. Miele, D. Rhodes, B. Vallone.
Organizing Committee:
F. Angelucci, G. Boumis, A. Di Matteo, C. Exertier, L. Federici, A. Fiorillo, G. Giardina, A. Ilari, L.C. Montemiglio, C. Savino, B. Vallone.

PROGRAMME

October 10^th, 2019
DAY 1 – Sapienza University of Rome, Sala Odeion

9.30-10.00	OPENING CEREMONY Prof. Eugenio Gaudio Rector of Sapienza University of Rome Prof. Maurizio Brunori Accademia Nazionale dei Lincei and Sapienza Emeritus Professor

9.30-10.00	OPENING CEREMONY Prof. Eugenio Gaudio Rector of Sapienza University of Rome Prof. Maurizio Brunori Accademia Nazionale dei Lincei and Sapienza Emeritus Professor

Session: CryoEM and biomedicine Part I

Chair Dr. Patrizia Lavia · IBPM-CNR, Rome – Italy

10.00-10.30	Prof. Ben F. Luisi Dept. of Biochemistry · Cambridge University (Cambridge, UK) Structure and Mechanism of Bacterial Multi-Drug Efflux Pumps The EMBO Keynote Lecture
10.30-11.00	Coffee Break
11.00-11.30	Prof. Daniela Rhodes Institute of Structural Biology · Nanyang Technological University (Singapore) Telomere Structure: A Combined EM Approach
11.30-12.00	Dr. Giovanna Scapin Merck (Kenilworth, NJ, USA) Insights into the Insulin Receptor Activation Mechanism from Single Particle CryoEM Analysis
12.00-12.30	Prof. Martino Bolognesi Dip. di Bioscienze · Universtà di Milano (Milan, Italy) Insight into Cas9 Allosteric Inhibition by the anti-CRISPR Protein AcrIIA6
12.30-13.30	Lunch break

10.00-10.30	Prof. Ben F. Luisi Dept. of Biochemistry · Cambridge University (Cambridge, UK) Structure and Mechanism of Bacterial Multi-Drug Efflux Pumps The EMBO Keynote Lecture
10.30-11.00	Coffee Break
11.00-11.30	Prof. Daniela Rhodes Institute of Structural Biology · Nanyang Technological University (Singapore) Telomere Structure: A Combined EM Approach
11.30-12.00	Dr. Giovanna Scapin Merck (Kenilworth, NJ, USA) Insights into the Insulin Receptor Activation Mechanism from Single Particle CryoEM Analysis
12.00-12.30	Prof. Martino Bolognesi Dip. di Bioscienze · Universtà di Milano (Milan, Italy) Insight into Cas9 Allosteric Inhibition by the anti-CRISPR Protein AcrIIA6
12.30-13.30	Lunch break

Session: CryoEM and biomedicine Part II

Chair Dr. Andrea Ilari · IBPM-CNR, Rome – Italy

13.30-14.00	Prof. Filippo Mancia Dept. of Physiology and Cellular Biophysics · Columbia University (NYC, USA) Membrane Protein Structural Biology with a Focus on Infectious Diseases
14.00-14.30	Prof. Beatrice Vallone Dip. Di Scienze Biochimiche · Sapienza Università di Roma (Rome, Italy) The Ferritin-CD71 Complex Maps Crucial Sites for Cell Entry
14.30-15.00	Prof. Alessandro Vannini The Institute of Cancer Research (London, UK) Unveiling RNA Polymerase III (Extra)Transcriptional Complexes with EM
15.0-15.30	Coffee Break

13.30-14.00	Prof. Filippo Mancia Dept. of Physiology and Cellular Biophysics · Columbia University (NYC, USA) Membrane Protein Structural Biology with a Focus on Infectious Diseases
14.00-14.30	Prof. Beatrice Vallone Dip. Di Scienze Biochimiche · Sapienza Università di Roma (Rome, Italy) The Ferritin-CD71 Complex Maps Crucial Sites for Cell Entry
14.30-15.00	Prof. Alessandro Vannini The Institute of Cancer Research (London, UK) Unveiling RNA Polymerase III (Extra)Transcriptional Complexes with EM
15.0-15.30	Coffee Break

Session: CryoEM sheds light on dynamical processes

Chair Prof. Maurizio Brunori · Accademia nazionale dei Lincei and Sapienza Emeritus Professor

15.30-16.00	Dr. Max Wilkinson Laboratory of Molecular Biology of the Medical Research Council (Cambridge, UK) How the Spliceosome Removes Introns from mRNA Precursors
16.00-16.30	Prof. Gebhard Schertler Dept. of Biology and Chemistry Paul Scherrer Institute (Villigen, CH) Cryo-EM Structure of the Light-Sensitive GPCR Rhodopsin in Complex with Heterotrimeric Gi
16.30-17.00	Prof. Giuseppe Zanotti Dip. di Scienze Biomediche · Università degli Studi di Padova (Padua, Italy) Nearly Atomic CryoEM Structure of a Flexible Filamentous Virus

15.30-16.00	Dr. Max Wilkinson Laboratory of Molecular Biology of the Medical Research Council (Cambridge, UK) How the Spliceosome Removes Introns from mRNA Precursors
16.00-16.30	Prof. Gebhard Schertler Dept. of Biology and Chemistry Paul Scherrer Institute (Villigen, CH) Cryo-EM Structure of the Light-Sensitive GPCR Rhodopsin in Complex with Heterotrimeric Gi
16.30-17.00	Prof. Giuseppe Zanotti Dip. di Scienze Biomediche · Università degli Studi di Padova (Padua, Italy) Nearly Atomic CryoEM Structure of a Flexible Filamentous Virus

October 11^th, 2019
DAY 2 – CNR, Sala Convegni

9.30-10.00	Welcome by the CNR president Prof. Massimo Inguscio Eulogy of Prof. Emilia Chiancone

9.30-10.00	Welcome by the CNR president Prof. Massimo Inguscio Eulogy of Prof. Emilia Chiancone

Session: CryoEM sheds light on dynamical processes

Chair Dr. Alberto Luini · IBP-CNR, Naples IItaly

10.00-10.30	Prof. Jürgen M. Plitzko Electron Microscopy Group · Max Planck Institute for Biochemistry (Munich, Germany) In Situ Structural Biology – Cryo-Electron Tomography of Cells and Tissue at Molecular Detail
10.30-11.00	Dr. Peter Rosenthal The Francis Crick Institute (London, UK) Imaging Viruses and Membrane Events by Cryomicroscopy
11.00-11.30	Coffee Break
11.30-12.00	Prof. Bram J. Koster Leiden University Medical Centre (Leiden, The Netherlands). Multi-Scale Light and Electron Microscopic Imaging in Biomedical Applications from Molecules to Microtissues
12.00-12.30	Prof. Andrea Mattevi Dip. di Biologia e Biotecnologie. Università di Pavia (Pavia, Italy) Structural Mechanisms of Nucleosome Recognition and Demethylation
12.30-13.30	Lunch break

10.00-10.30	Prof. Jürgen M. Plitzko Electron Microscopy Group · Max Planck Institute for Biochemistry (Munich, Germany) In Situ Structural Biology – Cryo-Electron Tomography of Cells and Tissue at Molecular Detail
10.30-11.00	Dr. Peter Rosenthal The Francis Crick Institute (London, UK) Imaging Viruses and Membrane Events by Cryomicroscopy
11.00-11.30	Coffee Break
11.30-12.00	Prof. Bram J. Koster Leiden University Medical Centre (Leiden, The Netherlands). Multi-Scale Light and Electron Microscopic Imaging in Biomedical Applications from Molecules to Microtissues
12.00-12.30	Prof. Andrea Mattevi Dip. di Biologia e Biotecnologie. Università di Pavia (Pavia, Italy) Structural Mechanisms of Nucleosome Recognition and Demethylation
12.30-13.30	Lunch break

Session: The methodology and its developments

Chair Prof. Filippo Mancia · Columbia University, NYC – USA

13.30-14.00	Prof. Amédée Des Georges Structural Biology Initiative, CUNY Advanced Science Research Center (NYC, USA) Harnessing the Power of Single-Particle Cryo-EM to Study Motion and Allostery in Ion Channels
14.00-14.30	Dr. Edoardo D’Imprima Max Planck Institute of Biophysics (Frankfurt, Germany) Emerging Challenges in High Resolution CryoEM: Interfaces Phenomena and Protein Denaturation
14.30-15.30	Talks from ThermoFisher, Jeol and Gatan
15.30-16.00	Coffee Break

13.30-14.00	Prof. Amédée Des Georges Structural Biology Initiative, CUNY Advanced Science Research Center (NYC, USA) Harnessing the Power of Single-Particle Cryo-EM to Study Motion and Allostery in Ion Channels
14.00-14.30	Dr. Edoardo D’Imprima Max Planck Institute of Biophysics (Frankfurt, Germany) Emerging Challenges in High Resolution CryoEM: Interfaces Phenomena and Protein Denaturation
14.30-15.30	Talks from ThermoFisher, Jeol and Gatan
15.30-16.00	Coffee Break

Round table on CryoEM: development of networks and infrastructures

Chair Dr. Willa Appel · New York Structural Biology Center NYSBC, NYC – USA

16.00-17.30	Panelists: Willa Appel, Martino Bolognesi, Giuseppe Cannone, Andrea Mattevi, Daniela Rhodes, Marco Rossi, Giovanna Scapin, Giancarlo Tria, Alessandro Vannini.

16.00-17.30	Panelists: Willa Appel, Martino Bolognesi, Giuseppe Cannone, Andrea Mattevi, Daniela Rhodes, Marco Rossi, Giovanna Scapin, Giancarlo Tria, Alessandro Vannini.

REGISTRATION IS CLOSED

No Participation fee is foreseen, but registration is compulsory, by September 15^th, 2019 at the latest. For further information and if you wish to present an E-Poster at the CNR Venue, please contact Beatrice Vallone at beatrice.vallone@uniroma1.it.

We encourage the participation of young scientists. To this aim a limited number of grants will be allocated by the scientific committee to support travel and accommodation expenses.

The grants are available for undergraduate or PhD students, as well as for post-doc researchers, less than thirty-five years old, thanks to the generous sponsorship of the Italian Crystallographic Association ad of the “Gruppo Proteine” of the Italian Society of Biochemistry. A preferential title will be the membership of one of the two Societies.

Participants interested to apply for a Grant shall register to the Conference, submitting an abstract, and explicitly apply for a Grant before September 15, by sending their Curriculum Vitae (max 2000 characters) to beatrice.vallone@uniroma1.it

VENUES

10^thOctober 2019
SAPIENZA Università di Roma
Aula Odeion
Piazzale Aldo Moro, 5, 00185 Roma RM
Museo Arte Classica · Ground Floor

GOOGLE MAP DIRECTIONS

11^thOctober 2019
CNR Consiglio Nazionale delle Ricerche
Sala Convegni
Via dei Marrucini, 00185 Roma RM (at the corner of Piazzale Aldo Moro)

GOOGLE MAP DIRECTIONS

Accomodation
Rome is a big city and can easily (and randomly) become very chaotic, this is why we strongly recommend that you book your hotel within walking distance from the Conference venue.
If you decide to stay in another district of Rome, our advise is to choose it near an underground station (B-line).
If you are planning to come in small (2-6 people) groups, also consider booking one of the many available apartments.

FIND AN ACCOMODATION

10^th and 11^th October 2019 – Cryo-Electron Microscopy in structural biology: paving the way towards precision biomedicine and biotechnology. A conference in memory of Emilia Chiancone.

Rome, Italy
10^thOctober 2019 · Aula Odeion Sapienza
11^th October 2019 · Sala Convegni CNR

PROGRAMME