Prometeus Internal Workshops on Knowledge Acquired and Knowledge Return Phase.
Date: 6 December 2023
Online Event
Workshops
These workshops provide an opportunity for researchers to share new skills/competence and knowledge acquired during the secondment. The results of the activities performed during the secondment will be described by Secondees and discussed among researchers.
Participation is restricted to Prometeus Partner Organization.
Registration
Register for the Workshops by filling out the form. Upon completion of registration you will receive the Zoom link to participate online.
1. Compound screening and synthesis for Protein Stabilization (10:00-11:30)
Chair: R. Ventura (ExtremoChem) and A. Boffi (MoLiRom)
| 10:00 – 10:20 | Cyrene solvent in biochemistry: protein stability & enzymatic reactions R. Piacentini Sapienza University of Rome (Italy) |
|---|---|
| 10:20 – 10:40 | Screening of synthetic glycoconjugates for protein stabilization E. Gugole Sapienza University of Rome (Italy) |
| 10:40 – 11:00 | Stabilization and activity tests of SRD5A enzymes I. Freda Sapienza University of Rome (Italy) |
| 11:00 – 11:30 | Discussion |
| 10:00 – 10:20 | Cyrene solvent in biochemistry: protein stability & enzymatic reactions R. Piacentini Sapienza University of Rome (Italy) |
| 10:20 – 10:40 | Screening of synthetic glycoconjugates for protein stabilization E. Gugole Sapienza University of Rome (Italy) |
| 10:40 – 11:00 | Stabilization and activity tests of SRD5A enzymes I. Freda Sapienza University of Rome (Italy) |
| 11:00 – 11:30 | Discussion |
2. Protein Production and Purification (13:00-14:50)
Chair P. Mariani (Università Politecnica delle Marche)
| 13:00 – 13:20 | Solubilization of human recombinant paraoxonase-2 D. Sartini Università Politecnica delle Marche, Ancona (Italy) |
|---|---|
| 13:20 – 13:40 | Expression and purification of GtrB in complex with its ligands to unveil the molecular basis of catalysis A. Romagnoli Università Politecnica delle Marche, Ancona (Italy) |
| 13:40 – 14:00 | Production and preliminary cryoEM studies on mycobacterial arabinofuranosyl transferases V. Almeida ITQB-NOVA, Oeiras (Portugal) |
| 14:00 – 14:20 | Design, expression and purification of recombinant ferritin Conjugates M.M. Rodrigues iBET, Oeiras (Portugal) |
| 14:20 – 14:50 | Discussion |
| 13:00 – 13:20 | Solubilization of human recombinant paraoxonase-2 D. Sartini Università Politecnica delle Marche, Ancona (Italy) |
| 13:20 – 13:40 | Expression and purification of GtrB in complex with its ligands to unveil the molecular basis of catalysis A. Romagnoli Università Politecnica delle Marche, Ancona (Italy) |
| 13:40 – 14:00 | Production and preliminary cryoEM studies on mycobacterial arabinofuranosyl transferases V. Almeida ITQB-NOVA, Oeiras (Portugal) |
| 14:00 – 14:20 | Design, expression and purification of recombinant ferritin Conjugates M.M. Rodrigues iBET, Oeiras (Portugal) |
| 14:20 – 14:50 | Discussion |
3. Protein Structure and Function (15:00-16:50)
Chair: B. Vallone (Sapienza University of Rome) and M. Archer (ITQB/UNL)
| 15:00 – 15:20 | Cryo EM experiments at CUNY-ASRC M.C. Cedri Università Politecnica delle Marche, Ancona (Italy) |
|---|---|
| 15:20 – 15:40 | Structure of the ErbB3-Neuregulin-Fab Complex G. Bulfaro Sapienza University of Rome (Italy) |
| 15:40 – 16:00 | Structure of the ferritin-CD71 complex by cryoEM B. Vallone Sapienza University of Rome (Italy) |
| 16:00 – 16:30 | Discussion |
| 15:00 – 15:20 | Cryo EM experiments at CUNY-ASRC M.C. Cedri Università Politecnica delle Marche, Ancona (Italy) |
| 15:20 – 15:40 | Structure of the ErbB3-Neuregulin-Fab Complex G. Bulfaro Sapienza University of Rome (Italy) |
| 15:40 – 16:00 | Structure of the ferritin-CD71 complex by cryoEM B. Vallone Sapienza University of Rome (Italy) |
| 16:00 – 16:30 | Discussion |
